【摘要】 本实验主要采用水提醇沉法提取甘谷红芪多糖,首先用DEAE-Sepharose Fast Flow阴离子交换柱层析进行分离得到其中的一种酸性多糖,后者再经Sepharose 6B Fast Flow凝胶层析纯化后浓缩、透析、冷冻干燥。琼脂糖凝胶电泳显色证明该组分为均一性多糖,再经过紫外、红外光谱分析以及酸水解产物薄层分析,分析结果表明该酸性多糖为杂多糖,其组成单糖可能分别为:木糖、葡萄糖醛酸或半乳糖醛酸、氨基葡萄糖盐酸盐或氨基半乳糖盐酸盐、葡萄糖醛酸内酯或半乳糖醛酸内酯,并且含有硫酸根。更多还原

【Abstract】 Crude polysaccharide was prepared from Hedysarum radix in Gangu of Gansu province by firstly aqueous extraction and then ethanol precipitation. An acidic polysaccharide was purified from the crude polysaccharide extract by DEAE-Sepharose Fast Flow ion exchange column chromatography followed by Sepharose 6B Fast Flow gel column chromatography and then was subjected to composition and structural analysis. Agarose gel electrophoresis pattern indicated that the acidic polysaccharides is of high purity and further was proved to be heterogeneous by ultraviolet spectroscopy and infrared spectroscopy. Thin-layer chromatogram showed that the monosaccharide compositions are xylose, glucuronic acid or galacturonic acid, galactosamine hydrochloride or galactosamine hydrochloride, glucuronolactone or galactonolactone with the presence of sulfate group.更多还原