【摘要】 为了获得富硒黑木耳菌丝,提高黑木耳菌丝的产量,改善黑木耳菌丝的品质,以亚硒酸钠作为硒源,用不同碳源有机物和氮源有机物进行黑木耳的深层发酵,研究pH值和温度对黑木耳深层发酵的影响。实验结果表明,黑木耳菌丝生长的最佳条件为:葡萄糖作碳源,豆饼粉作氮源,pH5.3,温度28℃。在此最佳条件下加入不同浓度的亚硒酸钠溶液进行培养,通过测定黑木耳菌丝的生物量,粗多糖含量和硒吸收率,表明亚硒酸钠浓度在60mg/kg以下时可提高黑木耳菌丝产量;在60～100mg/kg之间时会降低黑木耳菌丝产量;而在60mg/kg时,硒的吸收率达到最高。更多还原

【Abstract】 In order to gain selenium-rich Auricularia auricular, and improve its output and ameliorate its quality, this study investigated the optimum conditions for submerged fermentation of Auricularia auricular with sodium selenite as selenium source. The results showed that the optimum carbon source, nitrogen source, pH and fermentation temperature are glucose, bean cake powder, 5.3 and 28 ℃, respectively. Under these conditions, different concentrations of sodium selenite solution were added in the fermentation, and then the biomass of Auricularia auricular, output of crude polysaccharide and absorption rate of selenite were determined. It was indicated that the output of Auricularia auricula mycelium may be promoted when the concentration of sodium selenite solution is below 60 mg/kg, while the output is reduced when the concentration of sodium selenite solution is between 60 mg/kg and 100 mg/kg. Moreover, when the concentration of sodium selenite solution is 60 mg/kg, the absorption rate of selenium is the highest.更多还原