【摘要】 为研究沙蒿(Artemisia sphaerocephala Krasch)籽中的水溶性多糖成分,经热水提取、醇沉、脱蛋白得到水溶性沙蒿籽胶粗多糖CASPs(crude Artemisia sphaerocephala Krasch seed gum polysaccharides),CASPs经DEAE-Cellu-lose柱层析分离得到ASPI、ASPII、ASPIII、ASPIV四个多糖组分,ASPI经Bio-Gel P-150柱层析纯化后得到ASPI-A,ASPⅢ经Sephadex G-75柱层析得到ASPⅢ-A,ASPⅢ-B,ASPⅢ-C三个组分。气相色谱分析表明,ASPI-A由阿拉伯糖、甘露糖、葡萄糖及半乳糖组成,它们之间的摩尔比为1:2.84:4.89:1.92;ASPⅢ-A由木糖及微量的鼠李糖、阿拉伯糖、葡萄糖、半乳糖、葡萄糖醛酸及半乳糖醛酸组成。高效凝胶渗透色谱(HPGPC)分析表明,ASPI-A为均一性多糖,平均相对分子质量为5.42×104D;ASPⅢ-A的相对分子质量大于4×105D。免疫活性实验结果表明,与Con A合用时,ASPI-A和ASPⅢ-A在一定浓度范围内对小鼠脾细胞增殖反应具有一定的增强活性;ASPⅢ-A在较高浓度时可能具有一定免疫抑制活性。更多还原

【Abstract】 To investigate the components of water-soluble polysaccharides of Artemisia sphaerocephala Krasch seed gum, crude Artemisia sphaerocephala Krasch seed gum polysaccharides (CASP) were isolated through hot water extraction, ethanol precipitation and deproteinazation, and then were fractionated into ASPI, ASPII, ASPIII and ASPIV by DEAE-cellulose column chromatography. ASPI was purified by Bio-Gel P-150 column chromatography and one fraction (ASPI-A) was obtained. ASPIII was fractionated into ASPⅢ-A, ASPⅢ-B and ASPⅢ-C by Sephadex G-75 column chromatography. Monosaccha- ride analysis by GC showed that the ASPI-A is composed of arabinose, mannose, glucose and galactose with a molar ratio of 1: 2.84:4.89:1.92; The ASPIII-A is composed of xylose and slight rhamnose arabinose, glucose, galactose, glucuronic acid and galacturonic acid. High performance gel permeation chromatography (HPGPC) showed that the ASPI-A is homogeneous polysaccharide with an average relative molecular mass of 5.42×104 D and the relative molecular mass of ASPIII-A is more than 4×105 D. The immunological activity assay revealed that in the presence of Con A , ASPI-A and ASPIII-A can increase the splenocyte proliferation in mice in a certain range of concentration; ASPIII-A may have a certain immunosuppression activity in higher concentration.更多还原