【摘要】 建立一种以高效液相色谱法测定发酵液中表面活性素(surfactin)含量的新方法。采用COSMOSIL 5C18-AR-Ⅱ(4.6×250mm)色谱柱,以乙腈(含0.1%三氟乙酸)和水(含0.1%三氟乙酸)为流动相,流速0.84ml/min,梯度洗脱,紫外检测波长210nm,在20min内检测到表面活性素。结果表明,运用本方法在1～100mg/L范围内表面活性素浓度与积分面积之间具有良好的相关性(r=0.9998),精密度为1.5%,检出限为1mg/L,回收率达到91.36%。更多还原

【Abstract】 A new analysis method of surfactin content in fermentation broth of Bacillus amyloliquefaciens ES-2 by high performance liquid chromatography (HPLC) was established. The detection of surfactin was finished in 20 minutes at 210 nm on COSMOSIL 5C18-AR-Ⅱcolumn (4.6×250 mm) using acetonitrile (containing 0.1% triflouroacetic acid)-water (containing 0.1% triflouroacetic acid) (60:40, V/V) as mobile phase at the flow rate of 0.84 ml/min by gradient elution. The correlation coefficient between integral peak area and concentration is 0.9998 in the range of 1 to 100 mg/L. The precision, detection limit and spike recovery of this method is 1.5%, 1 mg/L and 91.36%, respectively.更多还原