【摘要】 研究一点红黄酮的分离提纯及其清除1,1-二苯基-2-苦基苯肼自由基(DPPH·)和羟基自由基(·OH)作用。经乙醇回流提取、聚酰胺柱层析分离提纯得到一点红黄酮;采用薄层层析对其进行定性分析;采用紫外-可见分光光度法测定其清除DPPH·和·OH的能力。结果表明,一点红黄酮清除DPPH·和·OH的IC50分别是4.8×10-3、0.53mg/ml,说明它具有清除DPPH·和·OH的作用。更多还原

【Abstract】 The total flavonoids of Emilia sonchifolia were extracted by ethanol refluxing and separated by polyurethane column, and their scavenging activities to 1,1-diphenyl-2-picrylhydrazyl (DPPH·) and hydroxyl radicals were measured by spectrophotometry. The results showed that the obtained products from Emilia sonchifolia are total flavonoids confirmed by thin layer chromatography, the separation effect of total flavonoids from Emilia sonchifolia is satisfying, and the total flavonoids present good scavenging activities to DPPH· and hydroxyl radicals with the IC50 values of 4.8×10-3 mg/ml and 0.53 mg/ml, respectively.更多还原