【摘要】 构建了一台基于串联式压电传感器的自动化微生物检测仪。对加入大肠杆菌的模拟牛奶样品进行检测,根据检测时间和样品中细菌浓度之间的线性关系,可以对样品中的细菌浓度进行计数,测定范围为10个/ml到106个/ml,检测时间3～15h。该方法的准确度与标准的平板计数法相当(r≥0.99),但是更灵敏、快速、简便。更多还原

【Abstract】 An automatic detection instrument based on tandem type piezoelectric biosensor was established,and then a model milk sample inoculated with Escherichia coli was tested by this instrument.Based on the linear relationship between frequency detection time(FDT) and bacterial concentration of milk sample,accurate numbers of counting bacteria were attainable.The results showed that target bacteria may be detected at concentrations ranged from 10 cell/ml to 106 cell/ml,and detection time is 3 to 15 h.The accuracy of this method is similar to the standard plate counting method(r≥0.99),but it is more sensitive,rapider and more convenient.更多还原