【摘要】 以鸡胸软骨为原料制备软骨粉,采用盐酸胍溶解去除非胶原成分后,添加胃蛋白酶酶解提取软骨粉Ⅱ型胶原。SDS-PAGE电泳和RP-HPLC色谱证实了所提取的软骨粉Ⅱ型胶原具有较高的纯度。氨基酸组成分析、紫外吸收光谱、FT-IR谱图、圆二色谱和差示扫描量热分析的测定结果表明软骨粉Ⅱ型胶原保持天然构象,结构没有受到破坏。更多还原

【Abstract】 The material powder was prepared from chick sternal cartilage,hydrochloric carbamidine was used to remove non-collagen components,and then pepsin was added to extract type Ⅱ collagen.The results of SDS-PAGE electrophoresis and RP-HPLC showed that the extracted type Ⅱ collagen has high purity.Analysis results of amino acid components,UV absor-bance spectrogram,infrared spectrum,circular dichroism(CD) spectrogram and differential scanning calorimeter(DSC) proved that type Ⅱ collagen from cartilage powder remains its natural structure.更多还原