【摘要】 本实验主要介绍了自制模拟微重力效应反应器(回转器)的工作原理、结构和特征,并用其进行螺旋藻光合自养分批培养,同时与摇瓶和发酵罐式光生物反应器对比。研究结果表明:在接种量为10%、光照强度为4000lx、温度为25℃的条件下培养9d,模拟微重力效应反应器效果最好,细胞干重为0.91g/L,是螺旋藻大规模培养的2.41倍,并且螺旋藻具有较好的细胞形态。更多还原

【Abstract】 In this study,the operation principle,structure and feature of simulated microgravity reactor were introduced mainly.Spirulinas platensis were cultured in phototrophic batches in three kinds of culture devices.The results showed that the simulated microgravity reactor(clinostat) for Spirulinas platensis culture is better than shake flask and fermenter light bioreactor,and its optimum conditions are as following:inoculation concentration 10%,illumination 4000 lx,25 ℃ and culture time 9 days.By this way,the production of Spirulina platensis(dw) is 0.91 g/L,which is 2.41 times as much as that by large-scale culture and the Spirulina platensis trichomes cultured in the simulated microgravity reactor have good cell morphology.更多还原