【摘要】 本实验使用一个压电体声波生物传感器对模拟样品中的结核杆菌减毒菌株H37Ra进行快速检测,在池系数为0.513cm和培养基稀释倍数为10倍时,传感器具有较高的灵敏度,并得到了细胞浓度与频率之间的线性方程;使用2%NaOH处理样品,能去除杂菌的干扰,但会使检测时间延长。更多还原

【Abstract】 Mycobacterium tuberculosis is the pathogenic microorganism of tuberculosis,and it could be infected by contaminated food. A piezoelectric bulk acoustic wave biosensor was used for rapid detection of M. tuberculos attenuated strain H37Ra in food samples. With the pool coefficient of 0.513 cm and the 10-fold diluted culture medium,it was found that the biosensor had a relatively high detection sensitivity,and a linear equation between cell concentrations and wave frequency was also constructed. It was also found that a pre-treatment with 2% NaOH solution could avoid the interference of mixed bacteria but prolong the detection period.更多还原