【摘要】 采用单因素试验和L9(34)正交试验设计,研究了料液比、提取时间、提取温度对石仙桃多糖提取率的影响,筛选出了最佳提取工艺。采用盐析法除蛋白、乙醇反复沉淀的方法纯化石仙桃多糖。结果表明,影响多糖提取率的主次因素为提取时间、料液比及提取温度;石仙桃多糖最佳提取工艺条件为:提取温度80℃,浸提时间5h,料液比1:40。在此工艺条件下,石仙桃多糖的得率为2.25%。纯化后的多糖经纯度鉴定为均多糖。更多还原

【Abstract】 Using the methods of single factor test and orthogonal design test of L9(34),the effects of various factors such as the ratio of sample to water,the extraction time and temperature on the extraction of polysaccharides in Pholidota chinensis Lindl were investigated and the optimal conditions were concluded. The polysaccharides were purified by ethanol precipitation and protein salting-out. The results showed that the main factors from the most important to the less affecting the extraction efficiency of polysaccharides are as following: extraction time,ratio of sample to water and extraction temperature. The optimal extracting conditions are established as following: extraction time 5 hours,ratio of sample to water 1:40 and extraction tempera-ture 80 ℃. Under the optimized parameters,the extraction efficiency of polysaccharides in Pholidota chinensis Lindl is 2.25%,and the obtained polysaccharides are identified as pure polysaccharides.更多还原