【摘要】 本实验利用超声提取集成丙醇-硫酸铵双水相体系对苦荞麦苗中总黄酮进行提取分离,研究丙醇-硫酸铵双水相的形成和提取条件对总黄酮得率的影响。结果表明,本方法可有效地从苦荞麦苗中提取总黄酮,提取率为1.86%,提取物中总黄酮含量为19.3%,明显高于回流提取法。提取物对脂质过氧化具有良好的抑制作用,最大抑制率为68.2%,方法操作简便、条件温和,环境友好。更多还原

【Abstract】 The ultrasonic extraction technology coupling with propyl alcohol-ammonium sulfate aqueous aqueous two-phase separation for extracting total flavonoids from tartary buckwheat seedling was studied. The results showed that under the optimum conditions,the extraction rate of total flavonoids is 1.86 % and their content in extract is 19.3 %,which is much higher than that extracted by circumfluence of solvent. Moreover,the total flavonoids extract could singificant1y inhibit lipid peroxidation (LPO) induced by ·OH free radical generation system. In a word,this method is simply,mild conditions and environmentally friendly.更多还原