【摘要】 戊二醛将伴刀豆球蛋白(ConA)和载体壳聚糖膜交联,然后利用ConA与葡萄糖氧化酶糖链的特异性结合作用,实现酶的定向固定化。定向固定化的最适条件为戊二醛浓度0.1%、ConA浓度0.02mg/ml、葡萄糖氧化酶浓度0.08mg/ml。定向固定化葡萄糖氧化酶的最适pH4.0、最适温度57℃,米氏常数K_m为15.84mmol/L,与游离酶及非定向固定化葡萄糖氧化酶比较,定向固定化葡萄糖氧化酶的最适pH值向酸性范围发生了偏移并有更宽的pH值适用范围,最适温度提高,与底物的亲和力较大。更多还原

【Abstract】 Concanavalin A (ConA),which has a very strong interaction with glycoprotein,was immobilized on preactivated glutaraldehyde-modified chitosan microspheres,and then oriented immobilization of glucose oxidase was carried out on the strong interaction.The optimal immobilization conditions are as follows:glutaraldehyde concentration 0.1%,ConA concentra- tion 0.02 mg/ml and glucose oxidase concentration 0.08 mg/ml.The optimum pH and temperature for orientedly immobilized glucose oxidsse are 4.0 and 57℃respectively,and its Michaelis constant (K_m) is 15.84 mmol/L.Compared with the free and the randomly immobilized glucose oxidases,the optimum pH of the orientedly immobilized glucose oxidase tends more acidic and its pH range is wider.Orientedly immobilized glucose oxidase also presents the higher temperature resistance and the better affinity to the substrate.更多还原