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黑玉米和黄玉米抗氧化提取物的抗氧化实验研究
Study on antioxidant activity of the yellow corn and black corn extracts
【摘要】 按极性大小对黄玉米和黑玉米中的抗氧化性物质进行溶剂超声提取,并对所得提取物进行清除DPPH.实验,得出黄玉米和黑玉米各自用不同溶剂提取物中抗氧化性最强的部分;以两种玉米提取物中抗氧化性最强的部分为抗氧化剂,进行邻苯三酚自氧化抑制实验和猪油自氧化抑制实验;对两种玉米提取物中抗氧化性最强部分进行总黄酮含量的测定。结果表明:黄玉米和黑玉米各自用不同溶剂提取物中抗氧化性最强的部分均为80%乙醇提取物;黄玉米乙醇提取物中总黄酮含量为2.0mg/g,黑玉米中总黄酮含量为6.4mg/g,三种抗氧化实验均说明黑玉米抗氧化性比黄玉米好,具有较好的清除自由基和抗氧化的能力。
【Abstract】 The different fractions of corn antioxidative extracts were obtained using solvents with different polarities.The antioxidative activity of these extracts were assessed for DPPH·radical scavenging activity to find the strongest antioxidative fractions in both corns.Taking the strongest antioxidative fractions as experimental objects,their antioxidiative activities were investigated by two different methods in scavenging superoxide anion(O-2·) generated by pyrogallol autoxidant system and restraining lard oxidation.Finally,the total flavonoids contents of the strongest antioxidative fractions were determined.The results showed that ethanol-water(80%,v:v)antioxidative extract fraction had the strongest antioxidative capacities among the five fractions in both corns.The total flavonoids contents of ethanolic extract were 2.0mg/g and 6.4mg/g for yellow corn and black corn,respectively.Three antioxidative experiments showed that the antioxidative activity of black corn was better than that of yellow corn.
【Key words】 black corn; yellow corn; antioxidant activity; DPPH·; pyrogallol; total flavonoids content;
- 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2009年07期
- 【分类号】R151
- 【被引频次】4
- 【下载频次】527