【摘要】 以分离自蒙古族发酵乳制品中的乳酸菌为研究对象,筛选得到一株可高产γ-氨基丁酸的乳酸乳球菌WH11-1。并通过正交实验对其发酵培养基进行优化,得出最佳培养基组成为:复合碳源质量分数为2%,其中葡萄糖与糊精质量比为3:1,复合氮源中蛋白胨与酵母膏的质量比为1:3,总碳氮比为1:2,盐酸吡哆醛为0.15%。采用优化后的培养基,32℃培养96h,发酵液中γ-氨基丁酸含量可达10.78g/L。更多还原

【Abstract】 Lc.lactis WH11-1 with high production of γ-amino butyric acid was screened objecting on strains isolated from Mongolian fermented dairy products,and its fermentation culture medium was optimized by orthogonal experiments.Results showed that optimized medium should be contained 2% total carbon,among which the ratio of glucose and alsace gum was 3:1,and the ratio of soy peptone and yeast extract was 1:3 in the total nitrogen,and the total carbon-nitrogen ratio was 1:2.Moreover the content of pyridoxal phosphate was 0.15% in this medium.The yield of GABA was 10.78g/L after cultivated in optimized medium for 96h at 32℃ by the strain.更多还原