【摘要】 以魔芋粉为诱导底物,分别从底物浓度、氮源的选择、氮源重组比例、氮源的浓度及接种量等方面进行乳白耙菌β-葡甘聚糖酶产酶条件优化,最后进行了产酶动力学分析。实验结果表明,最优的培养条件为:魔芋粉浓度为2%,以蛋白胨和硫酸铵为氮源,二者添加比例为1:2,混合氮源浓度为0.4%,接种量为10%。发酵4d,β-葡甘聚糖酶酶活达到48.1IU/mL,是未优化培养基条件下酶活的2.3倍。更多还原

【Abstract】 The producing conditions of β-mannan mannohydrolase of Irpex lacteus were optimized,including concentration of konjac power,choice of nitrogen resource,recombinant proportion of nitrogen resource,concentration of nitrogen resource and innoculation volume,at last the producing-enzyme kinetic was analyzed.The optimization conditions were obtained as follows:konjac power concentration was 2%,and peptone and ammonium sulfate were choose as nitrogen resource,and the recombinant proportion was 1:2,and nitrogen resource concentration was 0.4%,innoculation volume was 10%.Under this optimization conditions,the β-mannan mannohydrolase activity reached to 48.1IU/mL after fermentation for 4d,which was 2.3 times than the activity of control sample.更多还原