【摘要】 目的:建立了快速、灵敏地检测婴幼儿配方乳粉中叶酸含量的方法。方法:在配方乳粉中加入60%的高氯酸溶液去蛋白,取一定量的滤液经过含有C18填料的固相萃取小柱进行除杂,再经过高锰酸钾衍生后利用反相高效液相色谱测定叶酸的含量。流动相选用pH5.0的浓度为0.1mol/L的磷酸二氢钠溶液,流动相流速为1.0mL/min,经BDSC18色谱柱进行分离(柱温为30℃)。结果:方法在0.005～1.5μg/mL范围内线性良好,方法的回收率为91.7%～98.3%,RSD为2.5%。结论:应用本方法检测婴幼儿配方乳粉中的叶酸,具有操作简单、精密度高、重现性好、容易推广等优点。更多还原

【Abstract】 Objective:a quick and sensitive method was developed for the determination of folic acid(FA) in infant formula milk powder by RP-HPLC. Method:60% perchloric acid was added into infant formula milk powder to move protein and after solid-phase extraction(SPE)clean-up with C18 material,FA was determined by reversed phase high-performance liquid chromatography(RP-HPLC). pH 5.0 and 0.1mol/L sodium dihydrogen phosphate was used as mobile phase,the flow rate was 1mL/min. BDS C18 was used as separating column,column temperature was 30℃. Result:the linear range of the method was 0.005～1.5μg/mL. The average recovery was 91.7%～98.3%. The relative standard deviations(RSD) was 2.5%. Conclusion:the method had following advantages:simple,reliable and easy to extend.更多还原