【摘要】 建立一种测定芹菜中芹菜素含量的高效液相色谱法,测定湖南不同地区的芹菜中芹菜素含量。采用高效液相色谱法,色谱柱为Kromasil C18,流动相为乙腈和水(35∶65),流速1.0 mL/min,检测波长270 nm,柱温为35℃,进样量:10μL,检出限:18 ng/mL。在10～160μg/mL范围内呈良好的线性关系(r2=0.997 4),芹菜素平均回收率102%,(RSD=1.91%)。该方法操作简便、快速、分离度,灵敏度高,适用于芹菜素的测定。更多还原

【Abstract】 It was aim that the method to measure apigenin content of celery(Apium graveolens L.) was established by high performance liquid chromatography(HPLC).The apigenin contents of celery were determined in different area in Hunan.The determinate condition were as follows:the separation performed on Kromasil C18 column,mobile phase was acetonitrile-water(35∶65,V∶V),the flow rate was 1.0 mL/min,and measure wavelength was 270 nm,the column temperature is 35 ℃.injected sample volume 10 μL,limit of detection:18 ng/mL.The calibration curve of apigenin showed good linearity with peak area in the range of 10～160 μg/mL,(r2 =0.997 4),and the average recovery rate was 102%(RSD=1.91%).The advantages of this method are briefness,speediness,well separation,and high sensitive degree.更多还原