【摘要】 建立了同时测定环糊精中α-环糊精、β-环糊精、γ-环糊精3种成分含量的高效液相色谱法。方法采用色谱柱为SpherrigeL C6H5(5μm,300mm×3.9mm),流动相V(甲醇)∶V(水)=5∶95进行洗脱,流速为1.0mL/min,检测器为示差折光检测器,柱温40℃,进样量20μL。α-环糊精、β-环糊精、γ-环糊精的检出限分别为1.03,2.53,1.50μg/g,线性范围分别为2.0-207.9、11.5-230、3.9-194.2μg/mL,精密度相对标准偏差(n=6)分别为0.12%,1.13%,0.43%,加样回收率(n=6)平均分别为98.5%、98.0%、98.1%。该方法简便、灵敏、准确,可用于混合环糊精中α-,β-,γ-CD的含量测定和质量控制。更多还原

【Abstract】 An HPLC method for determination of three kinds of cyclodextrins,α-cyclodextrin,β-cyclodextrin,γ-cyclodextrin was developed. The system was : Spherrigel C6H5 column(5μm,300 mm×3.9 mm),methanol-water(5:95,v/v)as the solvent,the flow rate of 1.0 mL/min(column temperature 40℃)with the refractive index detector. The results were obtained that the detection limit of α-CD,β-CD,γ-CD were 1.03 μg/g,2.53 μg/g,1.50 μg/g,linear range of the three kinds of cyclodextrin were 2.0-207.9 μg,11.5-230 μg,3.9-194.2 μg respectively,relative standard deviation (n=6) were 0.12%,1.13%,0.43% and average recoveries (n=6) were 98.5%,98.0%,98.1% respectively. A specific,sensitive and simple HPLC method was established for the determination of mixed cyclodextrin and quality control.更多还原