【摘要】 为建立一种快速方便检测生物素含量的方法,采用生物素营养缺陷型菌株产氨短杆菌作为指示菌,研究了管碟法测定生物素含量的方法。研究结果表明,生物素浓度在15-95μg/L,平板指示菌生长圈直径大小与生物素浓度对数值呈一定线性关系,且线性良好,r=0.9933;平均回收率为101.0%,相对标准偏差4.984%-7.573%。更多还原

【Abstract】 In order to establish a convenient and rapid method to detect the content of biotin,the oxford plate assay system used to detect content of biotin was studied in this paper using Corynebacterium ammoniagenes,which is auxotrophic for biotin,as indicator bacteria.The results showed that a better linear relation between the diameter of the flat-panel indicator bacteria growth rings and the biotin concentration logarithm was obtained in the detected concentration range from 15 μg/L to 90 μg/L(r=0.993 3). The average recovery rate of this measurement was 101.0%and relative standard deviation(RSD) ranged from 4.984% to 7.573%.更多还原