【摘要】 为探讨海洋胶原肽(MCP)对痴呆性学习记忆下降的影响及作用机制,选用6月龄SAMP8小鼠为模型,分别喂饲含0、0.225%、0.450%和1.350%MCP的特殊加工饲料4个月,之后进行Morris水迷宫检测以观察小鼠的空间学习记忆能力、Nissl染色观察海马神经元的状况,同时检测海马区脑源性神经营养因子(BDNF)的表达状况。结果表明,0.44%和1.32%MCP干预可明显预防SAMP8小鼠学习记忆功能的下降;防止神经元过度丢失,促进脑源性神经营养因子的表达可能是其重要的作用机制。结果显示,海洋胶原肽具有抑制学习记忆下降的功能,可成为抗衰老相关功能食品的新选择。更多还原

【Abstract】 To explore the preventive effect and possible mechanisms of marine collagen peptide(MCP)on AD-related memory impairment,SAMP8 mice aged 6 months were divided into control group and three MCP treatment groups.The mice were fed with chow diet,0.225,0.450 or 1.350%(wt/wt)MCP respectively for 4 months.The Morris water maze was used to check the spatial learning and memory ability.The morphological changes of hippocampal tissue were estimated by nissl stain.Immunoblot analysis was used to measure the expression of brain-derived neurotrophic factor(BDNF).The results showed that spatial memory ability in 0.450 and 1.350% MCP-treated mice were significantly enhanced compared to SAMP8 control mice.The reduced apoptotic neurons and up-regulated expression of BDNF were involved in the mechanism of preventive effect of MCP on AD-related memory loss.These findings suggest that MCP could be a candidate for functional food to relieve memory deficits associated with aging.更多还原