【摘要】 采用脉冲电场液滴制备工艺,以海藻酸钠、壳聚糖为载体材料,纳米Fe3O4粉末为磁性材料,地衣芽孢杆菌为细胞模型,制备具有超顺磁性的微尺度载细胞微胶囊。以地衣芽孢杆菌的生长量为考察指标,考察不同磁含量、磁场引力、粒径和初始菌浓等因素的影响。实验结果表明,磁性材料Fe3O4的引入对地衣芽孢杆菌的生长没有影响。制备磁性固定化地衣芽孢杆菌微胶囊的最适条件为:Fe3O4含量0.003g/mL,初始接种密度为1.5倍菌浓(6.25×106个/mL),微囊的平均粒径为350μm,磁场环境的引入对地衣芽孢杆菌的生长无明显影响。论证了磁性壳聚糖/海藻酸钙微胶囊固定化细胞工艺进行在线分离,连续化操作的可行性。更多还原

【Abstract】 Taking chitosan and sodium alginate as the carrier,nano-Fe3O4 as the magnetic material,B.licheniformis as the cell model, magenetic microcapsule which has super-magnetism was prepared by the Impulsive Electrostatic Preparation process. Choosing the growth of B.licheniformis as the detecting target ,the optimum condition to prepare magnetic immobilized B.licheniformis were as follows : Fe3O4 concentration 0.003 g/mL; the initial inoculation density was 1.5 times(6.25×106 cells/mL); the average diameter of magnetic microcapsules were 350 μm. It also showed that the introduction of magnetic field had no significant effect .The study proved the possibility to separate on-line of the magnetic material and immobilized cell Fe3O4 chitosan/calcium alginate microcapsules and kept continuous operation.更多还原