【摘要】 采用固相萃取小柱对葡萄酒中的赭曲霉素A(OTA)进行浓缩处理,选择高效液相荧光法和超高效液相质谱联用法,分别测定葡萄酒中的OTA含量。采用HPLC-FLD法测定葡萄酒中OTA时,线性良好,R2=0.9997;经固相萃取小柱浓缩后,测定方法检出限可达到0.002μg/L;回收率为85.7%～102.6%;RSD(n=5)为4.5%。通过HPLC-FLD和UPLC-MS/MS测定OTA方法对比发现HPLC-FLD方法具有更好的灵敏度和检出限。更多还原

【Abstract】 In accordance with the nature of wine and OTA,we performed sample clean-up utilizing solid-phase extraction(SPE).The detection of wine OTA was accomplished using high-performance liquid chromatography(HPLC) combined with fluorescence detection(FLD),and ultra performance liquid chromatography(UPLC) combined with Tandem Quadrupole(MS/MS).Detection was validated by the clear linearity(R2=0.999 7) and the detection limit of OTA in wine were 0.004 g/L after concentration;recovery rate was 85.7%～102.6 %,and precision(RSD=4.5%,n=5).The comparison of HPLC-FLD and UPLC-MS/MS in terms of sensitivity and detection limit led to the conclusion that HPLC-FLD offered better results in the sub-ppb concentration level and higher sensitivity.更多还原