【摘要】 为了观察MK-801引发的精神分裂症模型小鼠脑内c-Fos和FosB/△FosB表达的差异,本实验选用雄性昆明小鼠,随机分为溶剂对照组、MK-801组(建立精神分裂症动物模型),采用免疫组化的方法检测模型动物脑内c-Fos和FosB/△FosB的表达,同时用免疫荧光双标对c-Fos和FosB/△FosB阳性的细胞进行定性分析。结果显示:精神分裂症动物模型组c-Fos蛋白的表达明显较FosB/△FosB广泛,几乎遍布全脑,而FosB/△FosB主要集中于前脑;在丘脑中线核群、板内核群、前核群和腹侧核群及脑桥核主要为c-Fos的表达,FosB/△FosB表达极少;在伏隔核、嗅结节、尾壳核、齿状回等核团主要为FosB/△FosB的表达,c-Fos表达极少。上述结果表明,c-Fos和FosB/△FosB的表达虽有重叠之处,但显示出明显的区域和强度的差异,且FosB/△FosB高度表达的区域与已知的精神分裂症相关脑区吻合,因此FosB/△FosB可能更适用于精神分裂症发病机制的研究。更多还原

【Abstract】 To observe the difference expression of c-Fos and FosB/FosB in the brain of schizophrenia model mice which is induced by MK-801,The male Kunming mouse are divided randomly into two groups:the experiment group receiving MK-801 injection to induce schizophrenia and the control group injected with dissolvent.The expression of c-Fos and FosB/FosB in the brain were detected by immunohistochemistry,and the positive cells expressing c-Fos and FosB/FosB were qualitatively analyzed of immunofluorescent staining.The expression of c-Fos was nearly detected in all the brain,while the FosB/FosB expression was detected mainly in the forebrain.The intensive expression of c-Fos was observed in midline nuclear group of thalamus,intralaminar nuclear group,anterior nuclear group,ventral nuclear group and pontine nucleus,without little FosB/FosB expression.The FosB/FosB positive staining was detected in nucleus accumbens septum,tuberculum olfactorium and dorsal caudate putamen,without little c-Fos positive staining.The results indicate the expression of c-Fos and FosB/FosB display the differences in area and intensity,and the area intensively expressing FosB/FosB is more nearly coincide with the area related to schizophrenia.So FosB/FosB is a better mark to research the pathogenesis of schizophrenia.更多还原