【摘要】 目的分析吡喹酮治疗前后小鼠脾细胞中CD4+CD25+Foxp3+T细胞的变化,探讨其与血吸虫感染后肝脏免疫病理反应的关系。方法54只BALB/C小鼠随机分为3组,阳性感染组、吡喹酮治疗组(感染后第6周顿服吡喹酮治疗)及阴性对照组。于感染后第3、6、9周分批处死小鼠,取脾单细胞悬液,经过常规表面染色、打孔、固定、胞内染色后,用流式细胞仪检测分析CD4+CD25+Foxp3+调节性T细胞的变化。结果感染组第3、6、9周脾细胞中CD4+CD25+Foxp3+T细胞的数量分别为(2.57±0.13)%、(1.77±0.21)%、(1.10±0.05)%,其含量逐步下降(P<0.05),治疗组分别为(2.61±0.12)%、(1.82±0.14)%、(1.76±0.11)%,经治疗后其含量变化差异无统计学意义(P>0.05),阴性组差异无统计学意义(P>0.05);CD4+CD25+Foxp3-T细胞和CD4+CD25-Foxp3+T细胞在各组中的含量差异均无统计学意义(P均>0.05)结论CD4+CD25+Foxp3+T细胞在日本血吸虫感染中具有免疫抑制作用,与血吸虫虫卵肉芽肿慢性病变具有相关性,吡喹酮可以通过抑制CD4+CD25+Foxp3+T降低以减缓血吸虫肉芽肿炎性病变的程度。更多还原

【Abstract】 Objective To analyse the dymamic changes of CD4+ CD25+ Foxp3+T cells before and after treatment of praziquantel in schistosomiasis mice and to explore the pathogenic reaction of mice ofter infected with schistosomiasis.Method 54 BALB/c mice were randomly derided into 3 groups i.e,praziquantel treated group,negative control group and positive control group,and the mice were killed at 3,6,9weeks after keing infected or freated.Then a spleen single cell suspension and a flow cytometry analysis of the CD4+ CD25+ Foxp3+T cells were made.Result The results showed that the CD4+ CD25+ Foxp3+T cells were declined remarkably from the 6 weeks after infection(P <0.05),but the CD4+ CD25-Foxp3+T cells and the CD4+ CD25+ Foxp3-T cells had no change(P >0.05).For the uninfected mice,all of the T cells had no change(P>0.05).For the treatment mice,it had no change after treatment(P >0.05).Conclusion It suggested that it was the CD4+ CD25+ Foxp3+T cells correlated closely to the murine schistosomiasis chronic hepatic fibrosis and the praziquantel could lighten the chronic inflammation of the liver through adjustment the CD4+ CD25+ Foxp3+T cells.更多还原