【摘要】 目的了解儿童临床分离肠球菌的耐药特征及其多重耐药与Ⅰ类整合子的相互关系。方法采用琼脂稀释法测定常用抗菌药物对152株肠球菌的最低抑菌浓度(MIC),用聚合酶链反应(PCR)检测肠球菌Ⅰ类整合子和Ⅰ类整合酶基因。结果屎肠球菌对氨苄西林、阿莫西林-克拉维酸、环丙沙星的耐药率分别为96.8%、95.2%和84.1%,粪肠球菌对上述3种抗菌药物的耐药率分别为23.6%、18.0%和49.4%,屎肠球菌的耐药率明显高于粪肠球菌(P<0.001);粪肠球菌中有2株对万古霉素的MIC为8μg/mL,粪肠球菌和屎肠球菌对替考拉宁均敏感。儿童多重耐药肠球菌发生率高达93.7%。屎肠球菌耐药模式以耐氨苄西林、红霉素、环丙沙星、利福平、四环素、高水平庆大霉素6种抗菌药物为主,占屎肠球菌的59%,粪肠球菌以耐四环素、红霉素、利福平、氯霉素、高水平庆大霉素5种抗菌药物为主,占粪肠球菌的26%。全部152株肠球菌未检测到Ⅰ类整合子,仅有5株检测到Ⅰ类整合酶基因。结论儿童肠球菌多重耐药十分严重,儿童肠球菌多重耐药与Ⅰ类整合子和Ⅰ类整合酶基因尚无明显关系。更多还原

【Abstract】 Objective To explore the resistant characteristic and the correlation between multidrug resistance and class I integron in Enterococcal isolates from children.Methods Mininal inhibitory concentrations(MIC) of 152 Enterococcal isolates were determined by agar dilution.Class Ⅰ integron and class Ⅰ integrase were detected by polymerase chain reaction(PCR).Results The resistant rate of Enterococci faecium(E.faecium) to ampicillin,amoxicillin-clavulanic acid,ciprofloxacin was 96.8%,95.2% and 84.1% respectively.The resistant rate of E.faecalis to ampicillin,amoxicillin-clavulanic acid,ciprofloxacin was 23.6%,18.0% and 49.4% respectively.The resistant rates of E.faecium to ampicillin,amoxicillin-clavulanic acid,ciprofloxacin were higher than E.faecalis(P<0.001).The MIC of vancomycin was 8 μg/mL in two E.faecalis.E.faecalis and E.faecium isolates were all susceptible to teicoplanin.The multiresistant rate to antibiotic in the Enterococci was 93.7%.The patterns of multiresistance to antibiotic in E.faecium were ampicillin,erythromycin,tetracycline,ciprofloxcin,rifampin,high level gentamicin.The patterns of multiresistance to antibiotic in E.faecalis were tetracycline,erythromycin,rifampin,chloramphenicol,high level gentamicin.Class Ⅰ integron was negative among all the Enterococcal isolates,only five Enterococcal isolates were tested class Ⅰ integrase.Conclusions The antimicrobial resistant status of the Enterococci is very serious.The multidrug resistance has no correlation to class Ⅰ integron and class Ⅰ integrase.更多还原