【摘要】 目的优化血清透明质酸(HA)时间分辨荧光免疫分析法(TRFIA)的反应条件,并探讨其临床应用价值。方法采用TRFIA检测血清中HA的含量,确定参与HA-TRFIA的各反应成分的浓度。结果最佳反应条件为包被浓度10 mg/L、HA结合蛋白(HABP)1∶500稀释、铕标记HA(Eu3+-BSA-HA)1∶200稀释。敏感性为5.17μg/L,批内变异系数(CV)为2.21%~3.78%,批间CV为3.73%~5.40%,平均回收率为100.4%。该法HA测定值与放射免疫测定值显著相关,且与临床结果一致。结论本研究建立的血清HA-TRFIA是一种灵敏准确的分析方法,适合临床应用。更多还原

【Abstract】 Objective To optimize the reaction conditions of the time-resolved fluoroimmunoassay(TRFIA)for hyaluronic acid(HA)detection in serum and explore its clinical application.Methods The serum levels of HA were detected by TRFIA.The different concentrations of the compositions in the HA-TRFIA system were set up.Results The optimum concentrations of goat anti-rabbit IgG,HA binding protein(HABP) and europium-labeled HA(Eu3+-BSA-HA)were 10 mg/L,1∶500 and 1∶200,respectively.The analytical sensitivity was 5.17 μg/L.The within-run and between-run coefficients of variation were 2.21%-3.78% and 3.73%-5.40%,respectively.The average recovery rate was 100.4%.The results had significant co-relationship with radioimmunoassay,and was consistent with the clinical diagnosis.Conclusions The competitive TRFIA for HA detection in serum is a sensitive and reliable assay.It would be suitable for clinical diagnosis.更多还原