【摘要】 目的探讨转化生长因子(TGF)-β1基因L10P多态与结直肠癌(CRC)遗传易感性的关系。方法采用Taq Man方法检测345例CRC患者及670名对照者的TGF-β1L10P基因型的分布。结果两组间TGF-β1L10P基因型分布的差异无统计学意义(P=0.213)。TNM分层分析结果显示,P是TNMⅠ～Ⅱ期CRC发病风险的遗传保护因子,与LL纯合子相比,LP杂合子的CRC发病风险显著降低至0.65(95%CI为0.44～0.95,P=0.027),PP纯合子的CRC发病风险显著降低至0.47(95%CI为0.28～0.78,P=0.004);将LP和PP基因型合并计算,则其CRC发病风险与LL基因型相比,降低至0.59(95%CI为0.41～0.85,P=0.005)。结论TGF-β1基因L10P多态可能是中国人群TNMⅠ～Ⅱ期CRC发病风险的一个遗传保护因素。更多还原

【Abstract】 Objective To investigate the association of the L10P polymorphism of transforming growth factor-beta 1(TGF-β1) gene and the genetic susceptibility to colorectal cancer(CRC) in a Chinese population.Methods The TGF-β1 L10P genotypes were determined by using the real-time PCR with TaqMan assay in 345 CRC patients and 670 sex-,age-matched controls.Odds ratios(ORs) for CRC and 95% confidence intervals(CIs) from unconditional logistic regression models were used to evaluate relative risks.Potential CRC risk factors,including sex,age,cigarette smoking,alcohol drinking,body mass index(BMI) and family history of colorectal cancer were included in the logistic regression models as a covariate in the multivariate analyses on genotype and CRC risk.Results The distribution of TGF-β1 L10P genotypes was not significantly different between the two groups(P=0.213).However,the P allele was significantly associated with a decreased risk for TNM stage Ⅰ—Ⅱ CRC as compared to the L allele.Compared with LL genotype,LP,PP and P-allele carriers(LP or PP genotypes) had a 0.65-fold(95% CI: 0.44—0.95,P=0.027),a 0.47-fold(95% CI: 0.28—0.78,P=0.004),and a 0.59-fold(95% CI: 0.41—0.85,P=0.005) decrease in risk of TNM stage Ⅰ—Ⅱ CRC,respectively.Conclusion The L10P polymorphism of the TGF-β1 gene may has a protective effect against TNM stage Ⅰ—Ⅱ CRC in Chinese population.(Shanghai Med J,2009,32: 885-887)更多还原