【摘要】 目的通过对体外培养软骨细胞的实验研究,探寻受体酪氨酸激酶抑制剂对软骨细胞在细胞分子水平的影响及其可能机制。方法对软骨细胞进行原代及传代培养,通过对培养细胞形态学及甲苯胺蓝染色观察,选择适合传代软骨细胞,应用三羟基异黄酮干预后1、2、3、4天RT-PCR、ICC及甲苯胺蓝染色观察法比较药物干预对体外培养软骨细胞Ⅰ、Ⅱ型胶原的转录和翻译水平及蛋白多糖的表达变化。结果20μg/mL及25μg/mL三羟基异黄酮干预可以抑制Ⅰ型胶原表达,同时增加Ⅱ型胶原及蛋白多糖的表达。结论三羟基异黄酮可以抑制脂多糖刺激诱导的软骨细胞反分化,对体外培养关节软骨细胞的正常表型具有一定的保护作用,其保护作用与三羟基异黄酮浓度呈现一定的相关性。更多还原

【Abstract】 Objective To explore the protective role of the receptor tyrosine kinase inhibitor in chondrocyte in vitro. Methods Reverse transcription-polymerase chain reaction(RT-PCR),immunocytochemistry(ICC) and toluidine blue staining were used to understand the changes of phenotype expression of collagen Ⅰ,Ⅱ and proteoglycan in chondrocyte in vitro. Results Genistein of 20 μg/mL and 25 μg/mL concentrations decreased the expression of collagen Ⅰ and increased the expression of collagen Ⅱ and proteoglycan (P<0.05). Conclusions This study showes that genistein can effectively inhibit the expression of collagen Ⅰ above certain concentration,and it can improve the expression of collagen Ⅱ and increase the synthesis of proteoglycan.更多还原