【摘要】 目的利用2型糖尿病不同病程人群空腹及葡萄糖口服应激模型,研究葡萄糖应激对人体自由基代谢指标变化的影响,探讨血糖波动与氧化应激水平和机体抗氧化代偿能力的关系,以探明自由基对糖尿病不同病程的介导规律。方法分为健康对照组(CO)、糖尿病前期组(DP)、糖尿病组(DM)和糖尿病并发症组(DC),每组选30例。氧化应激指标的测定:各组取空腹及口服葡萄糖液(GS)后2 h的肘静脉血各5 mL,检测超氧化物歧化酶(SOD)、丙二醛(MDA)、过氧化氢(H2O2)、维生素C(VC)和总抗氧化能力(T-AOC)。结果空腹氧化水平各组依次:DC>DM>DP>CO;还原水平除DP组SOD活力高于CO组,各组总体随糖代谢异常程度加剧呈下降趋势。葡萄糖应激2 h后各组自由基氧化还原水平排列类同空腹。GS干预前后比较,干预后各组H2O2水平都有不同程度提高,但还原水平各组有不同,CO与DP组在GS干预后,T-AOC水平比干预前都升高。随糖代谢异常程度加剧,氧化应激水平逐步增高,抗氧化能力逐趋下降,氧化损伤产物MDA增多。GS应激后,CO、DP组的总体抗氧化能力T-AOC显示代偿性增高,而DM、DC组无此效应。结论氧化应激在2型糖尿病的病理发展过程中起重要作用;CO与DP组所具的抗氧化代偿能力,提示对糖尿病的早期诊断和早期有效干预具有重要意义。更多还原

【Abstract】 Objective To detect the free radical metabolic changes in response to fast and glucose stress in different degree of type 2 diabetes mellitus,and to explore the relationship between blood sugar and oxidative stress/compensatory antioxidant capacity,thus to understand the role of free radicals in mediating diabetes mellitus. Methods Thirty prediabetic patients(DP group),30 diabetic mellitus patients(DM group),30 diabetes complications patients(DC group),and 30 healthy persons(CO group) were selected.Five mL vein blood sample was taken in diabetes mellitus patients after a fast or after 2 hours of oral administration with glucose.The oxidative stress parameters including SOD,MDA,H2O2,VC,VE and total antioxidant capability(T-AOC) were detected. Results In fasting serum,the oxidative level in each group was DC>DM>DP>CO.The reductive level in each group decreased upon the degree of diabetes,except that SOD in DP group was higher than CO group.After administrating glucose,the H2O2 level was increased in all groups with different degree.However,the changes of reductive level were different in 4 groups,T-AOC was increased in CO and DP groups after administrating GS.Upon the aggravation of the diabetes,the level of oxidative stress increased,the anti-oxidative ability decreased,and the oxidative product MDA increased.In response to glucose stress,the total antioxidant capacity(T-AOC) compensatory increased in CO and DP group,while in DM and DC group,T-AOC was not changed. Conclusions Oxidative stress plays an important role in the development of type 2 diabetes mellitus.The results that the anti-oxidative ability increased in control group(CO) and pre-diabetes(DP) group implies that early diagnose and interference are important in the treatment of diabetes mellitus.更多还原