【摘要】 采用制备型高速逆流色谱仪TBE-300A分离纯化北豆根药材中的活性生物碱类化合物。在实验过程中对溶剂系统和参数条件进行了系统的优化,获得较好的分离条件:溶剂系统为石油醚-乙酸乙酯-乙醇-水(1∶2∶1∶2,V/V/V/V),上相(有机相)为固定相,下相(水相)为流动相,反相模式洗脱;进样浓度20mg/ml;进样体积20ml;流速2.0 ml/min;转速850 r/min。通过一步分离,获得了四种高纯度的生物碱类化合物,经HPLC、MS和NMR鉴定,分别为蝙蝠葛苏林碱(101.47mg,96.7%),蝙蝠葛碱(155.68mg,95.6%),蝙蝠葛诺林碱((25.4mg,96.2%),蝙蝠葛新苛林碱(10.2mg,97.8%)。更多还原

【Abstract】 Preparative high-speed counter-current chromatography(TBE-300A) was used to separate and purify alkaloid compounds from the roots of Menispermum dauricum.In the process,we optimized the solvent system and the experiment parameters to be the following conditions: solvent system: petroleum ether-ethyl acetate-ethanol-water(1∶2∶1∶2,V /V /V /V),stationary phase: upper organic phase,mobile phase: lower aqueous phase;reverse phase model;sample concentration:20mg/ml;sample loading volume:20ml;flow rate: 2.0 ml/min;rotation speed: 850 r/min.Daurisolin(101.47mg,96.7%),dauricine(155.68mg,95.6%),daurinoline(25.4mg,96.2%)and dauricicoline(10.2mg,97.8%) were produced in only one step separation and their structures were identified by HPLC,MS,NMR.更多还原