【摘要】 目的 观察脾虚型肠易激综合征(IBS)模型WHBE兔的抗氧化活性的变化。方法 取WHBE兔和日本大耳白兔各12只，分别分成正常对照组6只、模型组6只，模型组采用湿热应激加灌服番泻叶煎剂的方法造成脾虚证肠易激综合征模型，造模结束后正常饲养10 d进行自然恢复，观察实验兔血清中的SOD、MDA、GSH-Px、NO和NOS活性水平的变化。结果 与正常对照组比较，模型组造模后WHBE兔和日本大耳白兔的血清SOD、GSH-Px活性水平显著降低(P<0.05,P<0.01);而血清MDA含量、NO和NOS活性水平均显著性升高(P<0.05,P<0.01);自然恢复10 d后，WHBE兔血清GSH-Px活性水平仍显著降低(P<0.05),MDA含量和NOS活性水平仍显著高于正常对照组(P<0.05);日本大耳白兔除NOS活性水平仍显著高于正常对照组(P<0.05)外，其它各项抗氧化指标均无显著差异(P>0.05)。结论 脾虚型肠易激综合征模型WHBE兔的抗氧化活性明显降低，且恢复较为缓慢，其症状与人的临床表现近似，因此WHBE兔可能适合用于人脾虚型肠易激综合征的研究。更多还原

【Abstract】 Objective To observe the change of anti-oxidation activity in WHBE rabbit with irritable bowel syndrome of spleen deficiency type. Methods Twelve WHBE(white hair and black ear) rabbits and 12 Japanese white rabbits were randomly divided into two groups respectively, with 6 rabbits in normal control group and 6 rabbits in model group. The irritable bowel syndrome of spleen deficiency type rabbits model was made by using the methods of moist heat, stress and stimulation adding with taking senna decoction, and each model group rabbits were recovered naturally for 10 days after the successful establishment. The levels of superoxide dismutase(SOD), the content of malondialdehyde(MDA), the activities of glutathione peroxidase(GSH-Px), nitric oxide(NO) and nitric oxide synthase(NOS) were determined. Results Compared with normal control groups, the activities of the serum SOD and GSH-Px were significantly decreased in WHBE rabbits and Japanese white rabbits after the successful establishment of the model(P<0.05, P<0.01), the content of MDA, the activities of NO and NOS were significantly increased(P<0.05, P<0.01). After 10 days nature recover, the activity of GSHPx in the natural recovery groups was still lower, while the content of MDA and the activity of NOS in the WHBE rabbits were all significantly higher than those in the normal rabbits(P<0.05). All the antioxidation indexes in Japanese white rabbits returned to the normal level, except for the GSH-Px activity was still higher than normal control group(P<0.05). Conclusion The anti-oxidation activity of WHBE rabbit of irritable bowel syndrome of spleen deficiency type were significantly decreased similar to clinic in human, and recovered slowly, so the WHBE rabbits may be suitable for the study of irritable bowel syndrome of spleen deficiency type in huaman.更多还原