【摘要】 目的 选用有较好多态性的微卫星位点对6种品系大鼠DNA多态性进行分析，以期建立一种准确可靠、高效可行的遗传监测方法。方法 选择大鼠不同染色体上的15个微卫星位点，应用PCR技术对6个品系大鼠进行了微卫星多态性分析。结果 9个微卫星位点均具有稳定扩增效果，在同一品系不同个体之间表现为单态性；在不同品系之间表现多态性，可用于大鼠遗传监测。结论 随着微卫星DNA多态性研究的不断深入，微卫星DNA可以取代生化标记分析法，广泛应用于大鼠的遗传监测中去。更多还原

【Abstract】 Objective Choose a microsatellite loci with better polymorphism to analyse DNA polymorphism of 6 strains of rat with a view to establishing a reliable and accurate, efficient and feasible genetic monitoring method. Method Fifteen rat microsatellite loci on different chromosomes were used to analyse the microsatellite DNA polymorphism among 6 rat strains by PCR methods. Result The nine microsatellites DNA could be amplified efficiently and displayed polymorphism among the various strains and no polymorphism in the same rat strains. Conclusion With the continuous deepening study of microsatellite DNA polymorphism of rat, the microsatellite DNA markers can replace the biochemical analysis, and be widely used in genetic monitoring.更多还原