【摘要】 利用电子断层三维重构技术对猪动脉内皮细胞(porcine aorta endothelial cell,PAEcell)胞膜小窝的三维结构进行了初步研究,发现胞膜小窝在细胞膜表面呈不均匀分布并在局部形成聚集,胞膜小窝膜内外表面都由宽度约14～16nm的条纹状结构所环绕,推测该条纹状结构主要由小窝蛋白和胆固醇构成,狭窄的胞膜小窝颈部区域存在高密度的丝状结构.三维结构显示胞膜小窝与纤维丝体网络(推测为微管网络)相互作用,暗示了细胞内吞可能的运输途径.更多还原

【Abstract】 A preliminary 3D structural analysis of caveolae from porcine aorta endothelial (PAE) cell has been done by electron tomography. Caveolae of PAE cell were distributed irregularly around the cell surface and aggregated locally to form cluster. The striated structure with a width of 14～16 nm around the caveolae can be seen at the inner or external side of the membrane, and more concentrated striations were found at the narrow neck. A three-dimensional structural model based on tomographic reconstruction shows that caveolae interact with potent microtubule network, suggesting a possible caveolae traffic path in the cell during endocytosis.更多还原