【摘要】 目的:对山西省浑源县不同购买批次的黄芪药材指纹图谱进行比较研究。方法:应用HPLC法,色谱柱:Thermo ODS(2.1mm×150mm,5μm);流动相:乙腈-水(3∶7);流速0.8mL/min;检测波长254nm;柱温:30℃;分析时间:60min;进样量:20μL。结果:山西省浑源县3种批号的黄芪药材有13个共有峰,其HPLC指纹图谱基本一致,但共有峰面积的比值有一定差异。结论:通过HPLC法建立了黄芪药材的指纹图谱分析方法,该方法稳定、可靠、重现性好,为快速鉴别区分不同来源的药材提供了科学的依据。更多还原

【Abstract】 Objective:To study comparatively on HPLC fingerprint spectra of 3 batches of Radix Astragali from Hunyuan County,Shanxi Province.Method:HPLC was performed on Thermo ODS column(2.1 mm×150 mm,5 μm)with acetonitrile-water(3:7)as mobile phase at flow rate of 0.8 mL/min.The column temperature was 30 ℃ and the detection wavelength was 254 nm.Analyzing time was 60 min and specimen handling quantity was 20 μL.Result:3 batches of Radix Astragali from Hunyuan County,Shanxi Province had 13 characteristic common peaks,but the ratios of common peaks area were different.Conclusion:Fingerprint spectra of Radix Astragali was constructed by HPLC.This method is stable,reliable,reproducible,and provides scientific basis for herbal discrimination.更多还原