【摘要】 目的:检测子宫腺肌病患者内膜组织中垂体肿瘤转化基因(PTTG)的表达,并分析其与基质金属蛋白-2(MMP-2)、基质金属蛋白酶组织抑制因子-1(TIMP-1)的相关性。方法:采用免疫组化SP方法检测35例子宫腺肌病组、在位内膜组和24例非子宫腺肌病患者正常内膜组中PTTG、MMP-2及TIMP-1蛋白的表达水平。结果:①PTTG在子宫腺肌病组、在位内膜组和正常内膜组间的表达差异有高度统计学意义(P<0.001),其中子宫腺肌病组和在位内膜组表达强度显著高于正常内膜组(P<0.001),子宫腺肌病组与在位内膜组间差异无统计学意义(P>0.05);②MMP-2在子宫腺肌病组和在位内膜组中的表达强度高于正常内膜组(P<0.05);③TIMP-1在子宫腺肌病组中的表达比正常内膜组低,两组相比差异有高度统计学意义(P<0.001);④PTTG与MMP-2的表达呈正相关,与TIMP-1的表达无相关性。结论:子宫腺肌病内膜中PTTG高表达,导致MMP-2表达上调,并可能影响TIMP-1的表达,从而使子宫内膜的增殖活性增强、侵袭力增高,致使子宫内膜向下浸润性生长,提示PTTG参与了子宫腺肌病的发生。更多还原

【Abstract】 Objective:To investigate expression of pituitary tumor transforming gene(PTTG),and its correlation with expression of matrix metalloproteinase-2(MMP-2) and tissue inhibitor of metalloproteinases-1(TIMP-1) in adenomyotic tissue.Methods:The expression of PTTG,MMP-2,and TIMP-1 were detected by immunohistochemistry using SP in 35 cases of the adenomyotic tissue and eutopic endometrium and 24 cases of normal endometrial tissue.Results:Expression of PTTG had statistic difference in the adenomyotic tissue,eutopic endometrium,and normal endometrium(P<0.001).PTTG expression in the adenomyotic tissue and eutopic endometrium was higher than normal endometrium(P<0.001),but there was no difference between them(P>0.05).The expression of MMP-2 in the adenomyotic tissue and eutopic endometrium was higher than that of in the normal endometrial tissue(P<0.05).TIMP-1 expression in the adenomyotic tissue was statistically lower than in the normal endometrium(P<0.001).PTTG expression had correlation with MMP-2,but not TIMP-1.Conclusions:Higher expressions of PTTG in adenomyotic endometrium will up-regulate MMP-2 and influence TIMP-1 expression.Therefore,endometrium enhances proliferation and invasion,finally result in the infiltrative growth of endometrium.PTTG may play an important role in the development of adenomyosis.更多还原