【摘要】 目的探讨慢性乙型肝炎患者肝组织中Foxp3表达及定位情况,并分析其与肝组织病理及血清学相关性。方法慢性乙型肝炎患者119例,对肝组织进行病理炎症活动度分级(G0~G4组)和纤维化分期(S1~S4组)。免疫组织化学法测定肝组织中Foxp3表达,并进行半定量分析。结果肝组织汇管区、小叶内Foxp3阳性表达G1~G4组均高于G0组(P均<0.05),肝组织汇管区Foxp3阳性表达量与炎症活动度呈正相关(r=0.561,P<0.01),但小叶区Foxp3各组阳性表达与炎症活动度无相关性(r=0.133,P>0.05)。Foxp3表达与不同肝纤维化分期、血清ALT水平及HBV DNA载量之间均无相关性(P均>0.05)。结论汇管区Foxp3的表达与病理炎症活动度具有相关性,迁延不愈的免疫损伤可能与机体缺少足够的调节性T细胞抑制有关。更多还原

【Abstract】 Objective To explore the expression and localization of Foxp3 in liver tissue with the patients of chronic hepatitis B(CHB),and analysis of the relationship and the immune mechanism with inflammatory activity and degree of fibrosis,ALT and HBV DNA load.Methods A total of 119 cases of CHB were obtained from the patients in liver biopsy,which were classified with inflammation activity grade and degree of fibrosis.The expression of Foxp3 of liver tissue were observed by immune histochemical techniques,and analyzing in half-ration.Results Compare with the G0 group,the scores of Foxp3 in portal tract and in the lobular had statistical significance(P<0.05).With the degree of inflammatory activity gradually increased,the expression of Foxp3 in portal tract was gradually enhanced(r=0.561,P<0.01).There is no correlation between the expression of Foxp3 in the portal tract and lobular of liver tissue and the degree of fibrosis,ALT,HBV DNA load(P>0.05).Conclusion The expression of Foxp3 with CHB have significant correlation with the inflammatory activity,the long-lasting immune injury may be correlated with the lack of sufficient inhibition of regulatory T cells.更多还原