【摘要】 目的探讨乏氧显像剂18F-氟赤式硝基咪唑(18F-FETNIM)在食管癌细胞ECa109乏氧监测中的应用价值。方法传代至2~3代的ECa109细胞,常氧环境下分别予以0、2、4、6、8 Gy X线照射,加入等量18F-FETNIM显像剂后分别于5、30、60、120、180 min用γ井型测量仪测定其放射性计数,计算摄取率。结果18F-FETNIM在ECa109细胞中的摄取,0 Gy组细胞的摄取率随时间延长而增高,180 min达最高,其余各组细胞随照射剂量增加,摄取率峰值逐渐前移,且峰值均高于0 Gy组。结论18F-FETNIM可用于食管癌细胞乏氧情况监测,为临床优化化疗方案提供依据。更多还原

【Abstract】 objective To investigate the monitoring value of hypoxic tracer fluorine-18-fluoroerythronitroimidazole(18F-FETNIM) in esophageal cancer ECa109 cells line.Method ECa109 cell line,which had been passaged 2-3 times and cultured under normoxic environment,were given different doses of X-ray irradiation(0 Gy、2 Gy、4 Gy、6 Gy、8 Gy),separately.The uptake of 18F-FETNIM in cell line were counted at 5 min,30 min,60 min,120 min,180 min separately after adding the hypoxia imaging agent 18F-FETNIM by Well γ-type measuring instrument.Result The cell line uptake rate of 0 Gy group increased continuously as the time pass and reached the peak at 180 min,while the other groups,the time to uptake peak were shortened and become shorter as the X-ray radiation dose enhanced step by step.All the peak uptake rate in X-ray radiation groups were higher than that in 0 Gy group.Conclusion The uptake of 18F-FETNIM for esophageal cancer cells can be used for the monitoring of hypoxia,in order to optimize the clinical programs provide a basis for radiotherapy and chemotherapy.更多还原