【摘要】 目的构建血管内皮生长因子(VEGF)短发夹RNA真核表达质粒(shVEGF),观察其对血管内皮瘤细胞系(EOMA)细胞体外增殖及动物实验的影响。方法构建真核表达质粒pGenesil-3-shVEGF并转染EOMA细胞,采用CCK-8法检测shVEGF对EOMA细胞增殖能力的影响。建立EOMA细胞裸鼠皮下移植瘤模型,分为对照组和shVEGF组,肿瘤出现后,隔日测量瘤体体积,并观察体积变化。结果转染48h后EOMA细胞增殖受到抑制。体内实验显示经shVEGF治疗后,裸鼠皮下移植瘤体生长缓慢,体积明显缩小。结论RNA干扰技术实现VEGF基因沉默,能明显抑制EOMA细胞增殖及体内肿瘤生长,提示shVEGF在血管内皮瘤、血管瘤等血管瘤样病变的生物治疗中可能有较好的应用前景。更多还原

【Abstract】 Objective To construct pGenesil-3-shVEGF and investigate its inhibitory effect on the proliferation of EOMA cells in vitro and in vivo.Methods The recombinant expression plasmid pGenesil-3-shVEGF was constructed and transfected into EOMA cells,the proliferation of cells was evaluated by CCK-8 assay.For in vivo assay,nude mice model bearing EOMA cells subcutaneous transplantation tumor were randomly divided into control group and shVEGF group,tumor volume was measured every other day after the emerge of tumor.Results The EOMA cell proliferation was inhibited 48 hours after the transfection.In vivo experiment showed that the tumor growth were delayed and the tumor volume reduced after the treatment of shVEGF.Conclusions RNAi-mediated inhibition of VEGF can significantly inhibit the proliferation of EOMA cell and growth of tumor in vivo,shVEGF may has a potential to be used in the treatment of hemangioendothelioma,hemangioma and so on.更多还原