【摘要】 目的探讨CD14基因-159C/T位点多态性和支气管哮喘(哮喘)的相关性及其对血浆IL-5的影响。方法选择正常对照组和哮喘组各150例,取外周血离心后,用酶联免疫吸附试验法测定血浆IL-5,用限制性片段长度多态性-聚合酶链反应方法检测CD14基因-159C/T位点多态性分布。结果对照组和哮喘组等位基因C、T分布有统计学差异(χ2=10.82,P<0.01),C等位基因与哮喘相关(C/T的OR=1.73,95%C I=1.25~2.39,P<0.01),两组基因型(TT、CT、CC)频率分布有统计学差异(χ2=9.73,P<0.01);同组内C等位基因携带者血浆IL-5高于非携带者,以CC基因型最高;同一基因型哮喘组IL-5高于对照组。结论CD14基因启动子-159位点多态性与哮喘相关,C等位基因与血浆IL-5升高相关。更多还原

【Abstract】 Objective To investigate the correlation between the polymorphism at-159 C/T locus in CD14 gene and asthma and its impact upon plasm IL-5 level.Methods 150 asthma subjects and 150 control subjects were chosen,after peripheral blood was centrifuged,plasm IL-5 level were tested by ELISA.Polymerase chain reaction and restriction fragment length polymorphism(RFLP-PCR) techniques were used to determine-159 C/T locus polymorphism.Results The distribution of C and T alleles were significantly different in the athma and control group(χ2=10.82,P<0.01),C allele was associated with asthma[OR(C/T)=1.73,95% CI =1.25～2.39,P<0.01].The genetype(TT,CT and CC) distribution was also significantly different in the two groups(χ2=9.73,P<0.01);in each group,subjects with C allele had higher IL-5 level than that in subjects without C allele,and the patients of CC genetype had the highest plasm IL-5 level;IL-5 level of patients with the same genotype in the asthma group were higher than that in the control group.Conclusions The-159 promoter polymorphism of CD14 gene may be associated with asthma.C allele is associated with higher plasm IL-5 levels.更多还原