【摘要】 目的探讨适度硬膜外低温(MEH)在大鼠脊髓损伤(SCI)后的神经保护作用及机制。方法选取16只大鼠建立SCI(T9节段)模型,随机分为观察组与对照组各8只,对照组不予任何处理,观察组设计MEH(30℃、48h)系统进行干预,7d后处死大鼠,行运动功能评分和斜面测试,HE染色及双重染色(TUNEL法和抗CC1),检测少突胶质细胞的凋亡及对小胶质细胞的抗炎作用。结果观察组运动功能显著改善,神经元和少突神经胶质细胞的细胞凋亡显著减少,小胶质细胞的活化显著抑制,与对照组相比,P均<0.05。结论MEH在SCI中具有显著的神经保护作用,其机制可能是抑制少突胶质细胞凋亡和抗炎作用。更多还原

【Abstract】 Objective To investigate the neuroprotective effect and mechanism of moderate epidural hypothermia(MEH) on spinal cord injury(SCI) in rats.Methods SCI model(T-9) was produced in all 16 rats,and then randomly divided into two groups.The control group was treated with no special measure,and the hypothermia group were interfered in MEH system(30 ℃,48 h).On the seventh postoperative day all rats were killed,motor function scores,inclined plane test and double stained(TUNEL stain and anti-CC1 immune stain)were used to evaluate the apoptosis of oligodendrocytes and the anti-inflammatory effects of microglia.Results Compared with the control group Motor function in the hypothermia group improved,the apoptosis of neurons reduced,the activation of the microglia inhibited(P< 0.05).Conclusions MEH have significant neuroprotective effect to SCI,its mechanism maybe inhibiting the apoptosis of the oligodendrocytes and the anti-inflammatory effects.更多还原