【摘要】 目的观察不同浓度三磷酸腺苷(adenosine triphosphate,ATP)对体外培养人鼻腔纤毛运动的影响。方法采用组织块培养法,应用高速数字化显微成像系统观察1μmol/L、10μmol/L和100μmol/L ATP对人鼻腔纤毛摆动频率(ciliary beatfrequency,CBF)的影响。结果Hanks平衡盐溶液(Hanks balanced salts solution,HBSS)对CBF无明显影响。②1μmol/LATP使CBF由(9.2±1.8)Hz升至(10.3±2.0)Hz(n=10)(P<0.05)。10μmol/L ATP使CBF由(9.5±1.7)Hz升至(11.5±2.0)Hz(n=10)(P<0.05)。③100μmol/LATP使CBF由(8.7±1.1)Hz升至(11.5±1.4)Hz(n=10)(P<0.05)。结论ATP可刺激体外培养人鼻腔纤毛摆动频率升高,在1~100μmol/L范围内,ATP对CBF的刺激呈浓度依赖性。更多还原

【Abstract】 Objective To investigate the effects of ATP on human nasal cilia movement.Methods Ciliary beat frequency(CBF)of cultured human nasal epithelial cells was measured by high-speed digital microscopy in ATP solution of different concentrations.Results CBF of cultured nasal epithelial cells exposed to HBSS showed no significant changes in 3 minutes(P>0.05);However in 1,10 and 100 μmol/L ATP solution,CBF increased significantly in 10 seconds and reached the apex,then it showed a sustained elevation in 3 minutes.In 1 μmol/L ATP solution,CBF increased significantly from(9.2±1.8)Hz to(10.3±2.0)Hz(n=10)(P<0.05).Similarly in 10 μmol/L ATP and 100 μmol/L ATP solution,CBF increased significantly when compared with samples in HBSS.Conclusion When 1,10 and 100 μmol/L ATP were added to the chamber,there were rapid increase in ciliary activity.In addition,there is concentration-dependent relation between CBF and ATP.更多还原