【摘要】 目的通过研究垂体腺瘤、胶质瘤、脑膜瘤、颅咽管瘤患者外周血NK细胞表面受体NKG2D的表达情况,分析探讨NKG2D表达与颅内肿瘤的关系,并结合临床资料,对垂体腺瘤患者NKG2D表达与临床因素关系进行了分析。方法通过红细胞裂解法,分离受检者外周血单核细胞,染色后利用流式细胞术检测NK细胞受体NKG2D的表达,并结合临床因素作比较分析。结果与正常人相比,胶质瘤、脑膜瘤、颅咽管瘤患者外周血NK细胞表面受体NKG2D表达无明显变化,垂体腺瘤患者外周血NKG2D的表达水平降低,并发现NKG2D的表达水平变化与垂体腺瘤患者视野缺损程度、出血等因素相关。结论在颅内常见肿瘤中,胶质瘤、脑膜瘤、颅咽管瘤患者外周血NK细胞受体NKG2D表达无明显变化,而垂体腺瘤患者外周血NK细胞受体NKG2D表达发生了变化,并且NKG2D表达水平与垂体腺瘤病情进展有一定的关系。更多还原

【Abstract】 Objective To explore expression levels of NKG2D on NK cells in patients suffering from pituitary adenoma,glioma,meningioma,and craniopharyngioma to obtain the relation ship between NKG2D-expressing NK cells and intracranial tumors. Methods Blood samples were lysed by FACS lysing solution,and expression of NKG2D on NK cells was determined by flow cytometry.Results The results revealed that expression of NKG2D was not obviously changed in glioma,meningioma,and craniopharyngioma but was decreased in pituitary adenoma.There were relations hips between the NKG2D expression and defect of visual field in pituitary adenoma.Conclusion In intracranial diseases,expression of NKG2D had no changes in glioma,meningioma,craniopharyngioma and was obviously changed in pituitary adenoma.NKG2D expression is related to the progression of pituitary adenoma.更多还原