【摘要】 目的对冠心病患者及正常对照者的3号染色体进行扫描,查找与冠心病关联的遗传位点。方法在3号染色体上采用间隔为10 cM(厘摩)遗传距离的23个微卫星遗传标记,分别对156例冠心病患者和450名正常对照者的DNA混合池样本进行基因组扫描,采用CLUMP软件对比患者组与对照组等位基因频率的差异。结果在D3S1278(3q13.31)位点和D3S1300(3p14.2)位点患者组与对照组的等位基因频率差异有统计学意义(P<0.001)。结论山东地区冠心病患者群体中存在与3号染色体q13.31和p14.2区域的关联,致病基因或调控因子可能位于其附近。更多还原

【Abstract】 Objective A screen on chromosome 3 in coronary heart disease(CHD) patients and normal controls from Shandong Province was performed to find the genetic loci associated with CHD.Methods A total of 23 microsatellite markers on chromosome 3 spaced at approximate 10 cM were used in screening of two separated DNA pool samples consisting of 156 CHD cases and 450 normal controls.Statistic analysis was performed by CLUMP software to compare the difference in allele frequency of each locus between the two pooled samples.Results Significant statistic differences were found at D3S1278 and D3S1300 loci between allele frequencies of patients and controls(P<0.001).Conclusion Two regions on chromosome 3 relate to CHD in Shandong Province and the susceptibility genes surrounding these regions need to be further studied.更多还原