【摘要】 为了提高尿素注射液治疗血管瘤的疗效,降低其副作用,我们研究制备了尿素免疫脂质体,并对其理化性质进行了研究。将血管内皮生长因子受体(VEGFR)的单克隆抗体作为偶联抗体,应用逆相蒸发法及交联法制备尿素免疫脂质体并测定包封率和偶联率,通过透射电镜观察尿素免疫脂质体的特征。结果表明:尿素免疫脂质体剂型稳定,肉眼观为乳白色混悬液,透射电镜下为大单层球形或近似球形结构,直径约150~200 nm,大部分可见到类核仁结构。其包封率约为54.4%,偶联率约为36.84%。医用尿素可以制成尿素脂质体,VEGFR单克隆抗体可作为免疫脂质体的偶联抗体,但需进一步提高包封率和偶联率。更多还原

【Abstract】 To improve the therapeutic efficency of treatment hemangioma with reducing the side effects of medical urea by preparing medical immunoliposomes and to study its physicochemical properties.Medical urea immunoliposomes were prepared through monoclonal antibody of vascular endothelial growth factor receptor being coupled to medical urea liposomes by reverse phase evaporation method and glutarldehyde method.The entrapment efficiency and the association ratio of the medical urea immunoliposomes were determined.Morphologic characteristics of medical urea immunoliposomes were investigated by transmission electron microscope.The results of gross appearance showed that the medical urea immunoliposomes were ivory white suspension and they were consisted of spherical or similar to spherical large unilamellar vesicles,and the particle size was about 150～200 nm by transmission electron microscope.The structure of nucleolus-like was observed.The entrapment efficiency of the medical urea immunoliposomes was up to 54.4%.The association ratio of the medical urea immunoliposomes was up to 36.84%.It is certain that the preparation of medical urea immunoliposomes is practicable and successful and monoclonal antibody of vascular endothelial growth factor receptor can act as coupled antibody of immunoliposomes.The entrapment efficiency and the association ratio should be raised.更多还原