【摘要】 采用RT-PCR方法对1株传染性支气管炎病毒(Infectious Bronchitis Virus,IBV)Chicken-SD-E-04的S1和N基因进行扩增、克隆测序。通过序列分析发现:该分离株的N基因相对较为保守,与参考毒株的氨基酸同源性在87.1%~91.2%;从N基因的遗传关系树上分析显示Chicken-SD-E-04与其他多数分离株间及疫苗株的遗传距离较远。而就S1基因而言,分离株与参考毒株的氨基酸同源性在73.2%~80.1%;从S1基因的遗传关系树上分析发现Chicken-SD-E-04与疫苗株的遗传距离较远,且处于不同的分支上。其结果说明这株山东分离株具有明显的地域性;而且也从分子水平上证实了目前山东流行毒株与传统疫苗株的遗传距离较远。更多还原

【Abstract】 The S1 and N genes of an infectious bronchitis virus(IBV) endemic strain Chicken-SD-E-04 were cloned and sequenced in the present study.Sequence analysis indicated that N gene was conservative and showed 87.1% ~91.2% of amino acid similarity with reference strains from GenBank.Based on the phylogenetic relationship of N gene,Chicken-SD-E-04 is least related to reference strains.When S1 genes were investigated,it demonstrated 73.2% ~80.1% of amino acid homology with reference strains.Chicken-SD-E-04 is phylogenetically far away from the immune strains which distributed in other regions.The above results verified that Chicken-SD-E-04 is obviously regional,and genetically distant from immune strains.更多还原