【摘要】 通过对4株红曲霉进行液体发酵研究,筛选出产酯化酶能力较高的红曲霉ZK,其发酵液中酯化酶活力为206.4 U.mL-1,再应用单因素和正交试验对其培养基配方进行研究,结果表明,液体发酵红曲霉的最佳培养基组成为:可溶性淀粉70.0 g.L-1、蛋白胨25.0 g.L-1、MgSO4.7H2O 1.0 g.L-1;连续进行4个批次的稳定性试验证明,以最佳培养基发酵,酯化酶平均酶活力为293.8 U.mL-1,酶活力提高了42.3%,为红曲霉的进一步开发利用提供了参考数据。更多还原

【Abstract】 To screen the high yield strain producing esterification enzyme,four strains of monascus were employed to optimize the liquid medium for fermenting esterification enzyme by single-factor test and orthogonal test in the current work.The result showed that the monascus ZK was the best among the four tested strains,with the activity of esterification enzyme 206.4 U·mL-1.The optimal liquid medium for esterification enzyme production contained 70.0g/L-1 soluble starch,25.0 g/L-1protein peptone,2.0 g/L-1NaNO3,and 1.0 g/L-1 MgSO4·7H2O.The average activity of esterification enzyme in optimal medium(293.8 U·mL-1)was 42.3% higher than that in basal medium among four times of constant tests.更多还原