【摘要】 从灰玫瑰青霉Penicillium griseoroseum发酵液中提取到1种具有抗菌活性的物质AF1,对其性质进行初步研究。发酵液经纱布过滤,离心后经5ku超滤膜超滤,经Sephadex LH20和HPLC分离纯化得到纯净物质AF1,并对其生物学活性进行研究。结果证明:AF1对β-内酰胺酶和酸碱稳定,对热有较好的耐受性,不是青霉素类抗菌物质;相对分子质量约为1465,结构中含有糖和氨基特征,对金黄色葡萄球菌的MIC为80μg/mL,对抗药金黄色葡萄球菌有效且不容易诱导细菌抗药性产生。更多还原

【Abstract】 An antimicrobial factor termed AF1 was isolated from culture broth of Penicillium griseoroseum.The filtered fermentation broth underwent centrifugation and ultrafiltration(membrane cutoff 5 kD).Then it was purified using Sephadex LH-20 and high-performance liquid chromatography(HPLC).The molecular weight of AF1 was 1465 Da.AF1 was stable to β-lactamase,hydroxylamine,acid,alkali and heat.Together,these data indicate that AF1 is a novel antibacterial factor which is different from penicillin.The molecular structure of AF1 shows characteristics of saccharide and amid.MIC of AF1 versus Staphylococcus aureus is 80 μg/ml,and AF1 is effective to drug-resistant S.aureus.It is difficult to induce bacterial resistant to AF1.更多还原