【摘要】 提出了一种简便、灵敏地测定银杏叶提取物中总黄酮含量的新方法。实验结果表明银杏叶提取物经酸性条件下加热水解,使黄酮苷转化为苷元再与Al3+配位,所形成的配合物具有较强的荧光,可以槲皮素为标准品,采用荧光光度法测定其总黄酮含量。在选定条件下,槲皮素的浓度与荧光强度成线性关系,线性范围:0.000 75~0.500 00μg/mL,检测限:0.45 g/mL(S/N=3),回归方程:If=7.799 48+63.210 86ρ×107(μg/mL),相关系数R=0.999 9(P<0.000 1),并用标准加入法进行回收实验和银杏叶提取物(GBE)中的总黄酮含量的测定,结果令人满意。更多还原

【Abstract】 A simple and sensitive new method for the determination of total flavonoids in ginkgo biloba extract was put forth.The experimental results indicated that the fluorescence intensity of ginkgo biloba extract(GBE) was greatly enhanced by heating in an acid medium and forming a complex with Al3+.A fluorimetric method for determination of total flavonoids in GBE was proposed by taking quercetin as the standard sample.Under the optimum conditions,a linear relationship was obtained between the fluorescence intensity and the concentration of quercetin in the range of 0.000 75～0.50 μg/mL(R=0.999 9; P<0.000 1),and the detection limit was 0.45 ng/mL(S/N=3).This method was applied for determining the total flavonoids in GBE,the recovery test was put forth by the standard addition method,and the expected results were obtained.更多还原