【摘要】 从山东省济南市南部山区土壤中分离得到1株具有广谱抗真菌活性的链霉菌SD-07菌株。根据形态特征、生理生化反应和16S rDNA序列分析结果,链霉菌SD-07鉴为定为纺锤链霉菌(Streptomyces netropsis)。分离纯化了SD-07产生的抗真菌活性成分并获得其结晶CA-SD07。抗真菌活性检测结果发现:CA-SD07对酵母菌、担子菌、接合菌等多种真菌具有广谱抗菌作用。对CA-SD07进行性质研究发现,抗真菌活性成分CA-SD07抗菌谱广且抗菌活性高(约等于2倍的两性霉素B),是戊烯和七烯混合的多烯大环内酯类抗真菌抗生素。更多还原

【Abstract】 Streptomyces sp.SD-07 was isolated from the soil of a mountain area in the south of Jinan,Shandong Province.This strain exhibited broad-spectrum and high antifungal activity.Based on the results of preliminary morphological, cultural,physiological and biochemical studies on this strain,and its 16S rDNA sequences,strain SD-07 was classified as Streptomyces netropsis.The antifungal compound CA-SD07 were extracted from the mycelia and then crystallized,and named as CA-SD07.CA-SD07 could inhibit many fungi such as Ascomycetes,Basidiomycetes and Zygomycetes.The data from ultraviolet-visible absorption and high-performance liquid chromatography / diode array detection(HPLC / DAD) indicated that CA-SD07 was a mixture of pentaene macrolide and heptaene macrolide antibiotics,and the major compound was pentaene macrolide.CA-SD07 has wider antifungal spectrum and higher antifungal activity(about two times Amphoteric B).更多还原