【摘要】 目的:观察青蒿琥酯对类风湿关节炎(rheumatoid arthritis,RA)的动物模型——胶原诱导性关节炎(colla-gen-induced arthritis,CIA)大鼠滑膜细胞的影响,探讨其对RA的作用机制。方法:雄性Wistar大鼠皮内注射Ⅱ型胶原,建立CIA大鼠模型,然后取其滑膜组织用胶原酶消化法进行原代培养。取3~5代的传代细胞,进行后续实验。流式细胞仪检测青蒿琥酯和甲氨喋呤对滑膜细胞生长周期的影响,电镜观察青蒿琥酯作用下滑膜细胞的形态。结果:青蒿琥酯和甲氨喋呤作用下细胞停留在G1期的比例比模型对照组明显增加(P<0.05),电镜下可以观察到青蒿琥酯引起滑膜细胞凋亡。结论:青蒿琥酯对RA的作用与抑制滑膜细胞的增殖,与诱导滑膜细胞凋亡有关。更多还原

【Abstract】 Objective:To observe the effects of artesunate on synoviocytes in collagen-induced arthritis rats(CIA)and to further investigate the possible mechanism.Methods:The model rats were immunized with type Ⅱ collagen to induce CIA.Synovial cells were isolated from the synovial issues and synovial cells were cultured by means of enzymatic digestion,we adopt the cell from third to fifth in this test.The apoptosis rate and cell cycle of synovial cells were examined by flow cytometer.The morphous of synovial cells were observe by electron microscope.Result:The rate of artesunate and MTX treated synovial fibroblasts in rats with CIA in G1 phase was significantly increased as compared with untreated gruop(P<0.05).That artesunate induced apoptosis of synovial cells was observed under electron microscope.Conclusion:It is suggested that the effects of artesunate on synoviocytes in CIA rats be concerned with the apoptosis of synovial fibroblasts.更多还原